Robert R. Puccinelli
EDUCATION
Ph.D. Bioengineering
UC Berkeley - UCSF Joint Graduate Program in Bioengineering
B.S. Physics
B.S. Biological Sciences
University of California, Merced
RESEARCH EXPERIENCE
Biosensor Design and Characterization
Joseph DeRisi, PhD; UC Berkeley - UCSF
Bioengineering Platform - Hardware Development
Rafael Gómez-Sjöberg, PhD; Chan Zuckerberg Biohub
High-Throughput Analysis of DNA-Protein Biophysical Interactions
Polly Fordyce, PhD; Stanford University
Influence of Nanoscale Topography on Actin Dynamics
Ajay Gopinathan, PhD; University of California, Merced
Influence of Nanoscale Topography and Electrical Stimulation on Stem Cell Fate
Kara McCloskey, PhD; University of California, Merced
TEACHING EXPERIENCE
BP 219
Instructors: Joseph DeRisi and Wallace Marshall
Teaching Staff: Rafael Gomez-Sjoberg, Eric Lam, Paul Lebel, Kevin Yamuchi, Joanna Cabrera, Robert Puccinelli
BIOE 301D/GENE 207
Instructors: Stephen Quake and Polly Fordyce
Teaching Staff: Kara Brower, Diego Oyarzun, Adam White, Robert Puccinelli
PUBLICATIONS
Google Scholar is the most up-to-date resource. Downloadable copies are provided below.
Remoscope: a label-free imaging cytometer for malaria diagnostics
Lebel P, …, Puccinelli R, et al.
Trans. R. Soc. Trop. Med. Hyg. 2025 Jul. DOI: 10.1093/trstmh/traf070
Open-source milligram-scale, four channel, automated protein purification system
Puccinelli RR, et al.
PLOS One. 2024 Feb. DOI: 10.1371/journal.pone.0297879
A handheld luminometer with sub-attomole limit of detection for distributed applications in global health
Lebel P, …, Puccinelli R, et al.
PLOS Global Public Health . 2024 Feb. DOI: 10.1371/journal.pgph.0002766
Comparison of SARS-CoV-2 Reverse Transcriptase Polymerase Chain Reaction and BinaxNOW Rapid Antigen Tests at a Community Site During an Omicron Surge
Schrom J, …, Puccinelli R, et al.
Annals Int Med. 2022 May. DOI: 10.7326/M22-0202
SARS-CoV-2 PCR and antibody testing for an entire rural community: methods and feasibility of high-throughput testing procedures
Appa A, CLIAHUB Consortium, et al.
Arv Pub Health. 2021 July 07. DOI: 10.1186/s13690-021-00647-8
Performance characteristics of a rapid SARS-CoV-2 antigen detection assay at a public plaza testing site in San Francisco
Pilarowski G, …, The CLIAHUB Consortium, et al.
J Infect Dis. 2021 Jan 04. DOI: 10.1093/infdis/jiaa802
Universal PCR and antibody testing demonstrate little to no transmission of SARS-CoV-2 in a rural community
Appa A, …, CLIAHUB Consortium, et al.
Open Forum Infect. Dis. 30 Oct 2020; DOI: 10.1093/ofid/ofaa531
Rapid deployment of SARS-CoV-2 testing: The CLIAHUB
Crawford ED, …, Puccinelli RR, et al.
PLOS Pathogens. 2020 Oct 28; 16(10):e1008966. DOI: 10.1371/journal.ppat.1008966
Identification of a polymorphism in the N gene of SARS-CoV-2 that adversely impacts detection by RT-PCR.
Vanaerschot M, …, CLIAHUB Consortium, et al.
J Clin Microbio. 2020 Oct 12. DOI: 10.1128/JCM.02369-20
Clinical features, diagnostics, and outcomes of patients presenting with acute respiratory illness: A retrospective cohort study of patients with and without COVID-19
Shah SJ, …, The CZB CLIAhub Consortium, et al.
EClinicalMedicine. 2020 Aug 25. DOI: 10.1016/j.eclinm.2020.100518
Community transmission of severe acute respiratory syndrome coronavirus 2 disproportionately affects the Latinx population during shelter-in-place in San Francisco
Chamie G, CLIAHUB Consortium, et al.
Clinical Infectious Diseases. 2020 Aug. doi: 10.1093/cid/ciaa1234
Diversification of DNA binding specificities enabled SREBP transcription regulators to expand the repertoire of cellular functions that they govern in fungi
Del Olmo Toledo V, Puccinelli R, Fordyce PM, Pérez JC
PLOS Genet. 2018 Dec 31; 14(12):e1007884. DOI: 10.1371/journal.pgen.1007884
Single-cell transcriptomics of 20 mouse organs creates a Tabula Muris
Tabula Muris Consortium; Overall coordination; Logistical coordination; Organ collection and processing; Library preparation and sequencing; Computational data analysis; Cell type annotation; Writing group; Supplemental text writing group; Principal investigators.
Nature. 2018 Oct; 562(7727):367-372. DOI: 10.1038/s41586-018-0590-4
An Open-Source, Programmable Pneumatic Setup for Operation and Automated Control of Single- and Multi-Layer Microfluidic Devices
Brower K, Puccinelli R, Markin CJ, Shimko TC, Longwell SA, Cruz B, Gomez-Sjoberg R, Fordyce PM.
HardwareX. 2018 Apr; 3:117-134. DOI: 10.1016/j.ohx.2017.10.001
Optimized Sequence Library Design for Efficient In Vitro Interaction Mapping
Orenstein Y, Puccinelli R, Kim R, Fordyce P, Berger B.
Cell Systems. 2017 Sep 27; 5(3):230-236.e5. DOI: 10.1016/j.cels.2017.07.006
PREPRINTS
Portable low-cost optical density meter
Puccinelli R, Cabrera J, Huynh E, Lebel P, Gomez-Sjoberg R
bioRxiv. 2021 May 17. DOI: 10.1101/2021.05.14.444207
Supervised self-collected SARS-CoV-2 testing in indoor summer camps to inform school reopening
Cooch P, CLIAHub Consortium, et al.
medRxiv. 2020 Oct 23. DOI: 10.1101/2020.10.21.20214338
POSTER PRESENTATIONS
Influence of Flanking Sequences in Transcription Factor - DNA Binding
Robert R. Puccinelli and Polly M. Fordyce
Stanford Department of Genetics Annual Retreat 2016
Resolving functional networks in a highly proficient enzyme
C. Markin, R. Puccinelli, C. Guegler, F. Sunden, I. AlSadhan, P. Fordyce, D. Herschlag
Stanford Department of Genetics Annual Retreat 2015
Modeling Actin Dynamics on Nanoscale Topography*
Robert R. Puccinelli and Ajay Gopinathan
Undergraduate Research Poster Competition, UC Merced 2014
*Certificate of Achievement awarded for best poster presentation
Roles for Electrical and Topographical Cues in the Maturation of Derived Cardiomyocytes
Robert R. Puccinelli, William S. Turner, Kara E. McCloskey
Student Research Poster Competition, UC Merced 2013
Combinatorial Effects of Nanoscale Topography and Electrical Stimulation in Stem Cell Fate
Robert R. Puccinelli, William S. Turner, Kara E. McCloskey
Sixth Annual Summer Research Symposium, UC Merced 2012
AWARDS/HONORS
NSF-GRFP Fellow, University of California, San Francisco
Graduation with Honors, University of California, Merced
Outstanding Student Award, University of California, Merced
Best Poster Presentation, UC Merced Research Week
Eagle Scout, Boy Scouts of America
INTERESTS
- Pathogen Detection and Identification
- BioMEMS
- Microfluidics
- Optical Instrumentation
- Remote Data Transmission
- Embedded Systems
- Instrumentation Programming and Control